GRS DNA Loading Buffer Blue (6X)

DNA loading Buffer Blue (6x) is a pre-mixed Loading Buffer for Agarose and Polyacrylamide gel electrophoresis, containing Bromophenol Blue as tracking dye.

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Categories:Buffers, DNA Ladders:GRS DNA Loading Buffer Blue (6X) is a pre-mixed Loading Buffer for Agarose and Polyacrylamide gel electrophoresis, containing Bromophenol Blue as tracking dye. EDTA is included to chelate Magnesium (up to 10 mM), stopping enzymatic reactions.

Contents:Solution of 10mM Tris-HCl pH 7,6; 60mM EDTA; 0,03% Bromophenol Blue; 60% Glycerol.

Applications:Preparation of DNA samples and markers for loading onto agarose and polyacrylamide gels. This Loading Buffer is a 6X concentrate: add 1μl of GRS DNA Loading Buffer Blue (6x) per 5 μl of DNA sample.

Dye Migration:Migration of the tracking dyes depend on the type of gel, concentration of the gel and the electrophoresis buffer. For example, on a 1% agarose gel the tracking dyes co-migrate with DNA fragments of the following sizes:

Bromophenol Blue

• TAE: ~ 500bp
• TBE: ~ 400bp
• VOLTage Fast: ~ 125bp

Specifications:Free of DNases and RNases.

Quality Control:Absence of endodeoxyribonucleases, exodeoxyribonucleases, and ribonucleases was confirmed by appropriate assays.

Storage:Store at room temperature or +4ºC for up to at least 12 months. For longer storage, store at -20ºC.

ORDER INFORMATION

• GLB01.0001 GRS DNA Loading Buffer Blue (6X) 1mL
• GLB01.0501 GRS DNA Loading Buffer Blue (6X) 5 x 1mL
• GLB01.2001 GRS DNA Loading Buffer Blue (6X) 20 x 1mL