Taq Polymerase

Catalog # :PCR-420-1MU

Please centrifuge briefly before opening (volume ≤2 ml).

Unit Definition: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmoles of dNTP's into an acid-insoluble form in 30 minutes at 70 °C using hering sperm DNA as substrate.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C,avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid

Concentration: 5 units/μl

Description:

The polymerase is recommended for routine PCR applications (up to 4 kb fragment length) or high throughput PCR.

The enzyme replicates DNA at 72 °C. It catalyzes the polymerization of nucleotides into duplex DNA in 5'→3' direction in the presence of magnesium. It also possesses a 5'→3' polymerization-dependent exonuclease replacement activity but lacks a 3'→5' exonuclease (proof-reading) activity.

Content:

Reaction Buffer and Nucleotides are not included. Please refer to our sections Buffers & Components and Nucleotides.

Taq Polymerase

5 units/μl Taq DNA Polymerase in 20 mM Tris-HCl, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, 50 % (v/v) Glycerol, pH 8.0 (25°C)

Assay Set-Up:

Before starting, thaw up all components and vortex thoroughly to ensure homogeneity.

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