GFP tag antibody 

Cat.#: 107296

Description:GFP tag Mouse Monoclonal antibody. Positive IF detected in Transfected HEK-293 cells. Positive IP detected in Transfected HEK-293 cells. Positive WB detected in Recombinant Protein.

Tested applications:ELISA, WB, IP, IF

Species reactivity:Tag

Alternative names:CFP antibody; eGFP antibody; eYFP antibody; GFP antibody; GFP tag antibody; YFP antibody

Isotype:Mouse IgG2a

Preparation:This antibody was obtained by immunization of GFP tag recombinant protein (Accession Number: AVU73901). Purification method: Protein A purified.

Clonality:Monoclonal

Formulation:PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions:Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:2000-1:20000

IP: 1:1000-1:10000

IF: 1:20-1:200

Background:Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYPF tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.

References:

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Das A, Gajendra S, Falenta K. RalA promotes a direct exocyst-Par6 interaction to regulate polarity in neuronal development. Journal of cell science. 127(Pt 3):686-99. 2014.

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