LE Agarose 

Catalog#:PCR-269

For general laboratory use.

Shipping: shipped at ambient temperature

Storage Conditions: store at ambient temperature

Shelf Life: 24 months

Form: fine homogeneous powder

Color: white

Applications:Nucleic acid analytical and preparative electrophoresis

Blotting:Protein electrophoresis such as radial immunodiffusion

Description:

LE Agarose is a general use agarose for routine nucleic acid analytical and preparative electrophoresis of fragments between 200 - 20000 bp, blotting and protein electrophoresis. This agarose has a low electroendosmosis value (EEO), ensuring high electrophoretic mobility of nucleic acids. Because of the high gel strength, easy-to-handle gels can be prepared with this agarose at concentrations as low as 0.4 %. LE Agarose has an exceptionally low fluorescence background.

Specifications:

Moisture:≤ 10 %

Ash:≤ 0.4 %

Electroendosmosis:0.05 - 0.13

Sulphate:≤ 0.1 %

Clarity 1.5 % (NTU):≤ 3

Gel Strength 1 %:≥ 1200 g/cm2

Gel Strength 1.5 %:≥ 2500 g/cm2

Gelling Temperature 1.5 %:36 ± 1.5 °C

Melting Temperature 1.5 %:88 ± 1.5 °C

Functional tests

DNase/RNase activity:none detected

DNA resolution > 1000 bp:finaly resolved

Gel background:very low

Recommended Agarose Concentrations:

Size Range(base pairs):Final AgaroseConcentration[%]1x TAE Buffer

1000 - 20000:0.60

500 - 12000:0.80

300 - 8000:1.00

200 - 6000:1.20

100 - 3000:1.50

100 - 2000:2.00

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