Ep-CAM / CD326 Recombinant Monoclonal Mouse Antibody (rVU-1D9)

Product Description

This antibody reacts with the first EGF repeat in the extracellular domain of Ep-CAM. It is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email order@biotium.com to inquire about stock status and lead times before placing your order.

Product Attributes

Antibody number:#1820

Antibody reactivity (target):CD326, Ep-CAM

Antibody type:Primary

Host species:Mouse

Clonality:Recombinant Monoclonal

Clone:rVU-1D9

Isotype:IgG1, kappa

Molecular weight:40-43 kDa

Synonyms:Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1)

Human gene symbol:TACSTD1

Entrez gene ID:4072

SwissProt:P16422

Unigene:542050

Immunogen:Small cell lung carcinoma cells

Antibody target cellular localization:Exosomes/EVs, Plasma membrane

Species reactivity:Human

Verified antibody applications:Flow (surface) (verified), IF (verified), IHC (FFPE) (verified)

Expected antibody applications:Exosome staining (verified for non-recombinant clone), WB (published for clone)

Antibody application notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, Immunohistology (formalin): 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Western blotting 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control:HT29 cells or breast tumor

Shipping condition:Room temperature

Storage Conditions:Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Regulatory status:For research use only (RUO)

Antibody/conjugate formulation:Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Shelf life:Guaranteed for at least 24 months from date of receipt when stored as recommended

Antibody research areas:Cancer, Cell adhesion, Exosomes/EVs

Cell/tissue expression:Epithelial cells

Tumor expression:Adenocarcinoma

Product origin:Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

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