2X TSS Bacterial Transformation Kit for Fast Preparation of Competent E.coli Cells

Transformation and Storage Solution (TSS) is used for the preparation of competent E. coli cells in a single step and to transform the cells without heat-shock. Amid Biosciences' TSS is optimized version of solution described initially by Chung et al. (1) and contains DMSO, PEG, Mg2+ salts, along with proprietary additives. This solution allows rapid preparation of the competent cells with typical transformation efficiencies that range from 10^5 to 10^7 cfu/µg of DNA, however, the transformation efficiencies up to 10^8 can be obtained for some E. coli strains.

For applications that require high efficiencies (e.g. library preparations, transformation of ligation reactions), Amid Biosciences’ selection of ready-to-use Chemically Competent or Electrocompetent Cells would be a good choice.

Benefits:

Fast preparation of competent cells.

Suitable for various E. coli strains (DH5a, HB101, JM109, XL1-Blue, and etc.)

Transformation cells without heat-shock.

Catalog # TSS-BTK-201

Transformation efficiencies range from 10^5 to 10^7 cfu/µg of DNA (up to 10^8 can be obtained for some E. coli strains)

Kit Components & Storage:

2X TSS: 10 ml. Store at -20 °C

SOC medium: 20 ml. Store at -20 °C or 4 °C

Control pUC19 plasmid DNA: 100 ng. Store at -20 °C

Fast Preparation of Competent Cells - TSS Protocols - Manual and Description

Shipping: Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates.

Reference:

Chung et al., One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. (1989) Proc. Natl. Acad. Sci. USA 86: 2172.

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