Tumor Cell Lines-KHYG-1

KHYG-1 was established 1997 from the peripheral blood of a 45-year-old woman with aggressive natural killer cell (NK) leukemia. Human natural killer cell line with a p53 point mutation as a model for p53-associated leukemogenesis and a model for differentiation of NK/T cells.

Product Code:KHYG1; KHYG

Species:Human

Cat.No:ABC-TC0506

Product Category:Tumor Cell Lines

Size/Quantity:1 vial

Cell Type:Lymphocyte-like

Shipping Info:Dry Ice

Growth Conditions:37 ℃, 5% CO2

Source Organ:Peripheral Blood

Biosafety Level:1

Storage:Liquid Nitrogen

Product Type:Human Leukemia Cell Lines

Description

KHYG-1 is a human natural killer (NK) leukemia cell line derived from the peripheral blood of a 45-year-old woman with aggressive NK cell leukemia. This cell line exhibits the morphology of large granular lymphocytes, characterized by a large nucleus, coarse chromatin, prominent nucleoli, and abundant basophilic cytoplasm containing many azurophilic granules. Immunophenotype of KHYG-1 includes markers such as CD2+, CD7+, CD56+, and CD122+, while it is negative for CD1, CD3, CD16, and CD57. KHYG-1 harbors a point mutation in exon 7 of the p53 gene, a mutation also found in primary leukemia cells, making it an ideal model for studying p53-associated leukemogenesis. KHYG-1 cells demonstrate high cytotoxicity against various leukemia cell lines, including EM2, EM3, and HL60, and this cytolytic activity is thought to be mediated by activation receptors such as NKp44, NKG2D, and phosphorylated ERK2. They are valuable for research into NK/T cell differentiation and leukemia therapy.

Application

KHYG-1 provides a valuable model for studying the biology of normal and malignant NK cells, and leukemogenesis. NK cells have been considered promising candidates for adoptive immunotherapy of malignancies, however, the endogenous NK and lymphokine-activated killer cells show limited cytotoxic activity. Through the investigation of enhanced cytotoxicity of permanent NK cell lines, such as KHYG-1, the limitation of autologous NK cells may be overcome, and NK cells may achieve enhanced cytotoxic potential clinically.

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