Phage Display SS320 Chemically Competent E. coli Cells 

The E. coli strain SS320 is a non-amber suppressor strain (sometimes called MC1061F′) prepared as high transformation efficiency (≥ 1 × 10^9 cfu/μg) chemically competent cells for phage display library screening. The SS320 strain harbors an episome that contains the F′ pilus that is required for bacteriophage entry to allow amplification of phage libraries. This episome also contains the tetracycline resistance gene, so cells should be grown in the presence of tetracycline to maintain the F′ pilus.

Features and Benefits:

Transformation efficiency > 1 X 10^9 transformants /µg pBR322

Useful for preparation of phage display libraries

Fast-growing

Tetracycline resistant

Catalog # SS320-201

Transformation efficiency: > 1 X 10^9 transformants /µg pBR322

Genotype: hsdR mcrB araD139 Δ(araABC-leu)7679ΔlacX74 galUgalK rpsL thi[F´ proAB+lacIqlacZΔM15 Tn10 (tetr)]

Storage: at -80°C

International Shipping: Product requires shipping on dry ice.

Reference:

Frei J. and Lai J. Protein and Antibody Engineering by Phage Display. Methods Enzymol. 2016; 580: 45–87.

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