KYSE-140

Cat.No.: CSC-C0404

Species: Human

Source: esophageal squamous cell carcinoma

Morphology: adherent cells growing as monolayer with the tendency to pile up

Culture Properties: monolayer

Cat.No.CSC-C0404

DescriptionDerived from the moderately differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 54-year-old Japanese man prior to treatment (depth of invasion was a1); described to carry p53 mutation

SpeciesHuman

Sourceesophageal squamous cell carcinoma

Recommended Medium90% RPMI-1640 + 10% h.i. FBS

Culture Propertiesmonolayer

Morphologyadherent cells growing as monolayer with the tendency to pile up

KaryotypeHuman hyperdiploid/hypotriploid karyotype with 19% polyploidy - 59(56-64)<2n>Xder(X), -Y, add(X)(p1?), del(1)(q32), add(1)(q43), i(3q), add(2)(p2?), del(3)(q22), add(4)(p11), del(4)(q11), add(5)(q1?)x1-2, ins(6;?)(q1?), add(7)(q35)x2, add(8)(q24), del(9)(

Quality ControlMycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization assays

Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cy

Storage and ShippingFrozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation GuidanceIf you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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