Babesia spp. qPCR Kit 

MD08071

Designed for the in vitro detection of Babesia spp. qPCR assay criteria are met, but PC is not meant for quantification.

Details

Babesia is an intracellular protozoan parasite that infect the red blood cells of a wide range of hosts, primarily mammals, including domestic animals and humans. Transmission typically occurs through the bite of infected ticks. In rare cases, it can also occur through blood transfusions from infected donors or, in some animals, from mother to offspring during pregnancy. The risk of infection increases in regions with high tick populations, especially during warmer months when ticks are most active.

Babesia spp. qPCR Kit is designed for the in vitro detection of Babesia genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Babesia spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this genus. Based on a comprehensive bioinformatic analysis with all reference data within the NCBI database the primers and probes sequences have very high (>95%) homology with a broad range of Babesia genomes of the following species: B. felis, B. gibsoni, B. microti, B. canis and B. divergens. Other closely related genus are not detected.

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.

Shipping Conditions:Room Temperature

Storage Conditions:-85 °C to -15 °C

Format:Lyophilized

Product Category:Predesigned qPCR assays

Compatibility:All standard Real-time thermal cyclers

Target Species:Babesia spp.

Sample Material:DNA

Protocol time:Approx. 60 min (from extracted NA to result)

Number of targets:Duplex

Internal Control:No

Detection Method:Probe Based

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