Mix-n-Stain™ Nanobody Thiol Labeling Kits

Label 5-50 ug of VHH (also known as Nanobodies®) containing a single free cysteine with Biotium’s bright and photostable CF® Dyes, with no purification after labeling.

PRODUCT DESCRIPTION

Mix-n-Stain™ Nanobody Thiol Labeling Kits are designed for labeling VHH (also known as Nanobody® or single-domain antibodies) that contain a single free cysteine. The labeling is done within 2 hours in ambient conditions, and requires minimal hands-on time and no purification.

Note: This kit is for labeling VHH via a single cysteine (1x Cys) only. Please see our Mix-n-Stain™ Nanobody Labeling Kits for standard labeling of VHH, or view our full selection of labeling kits for labeling IgG antibodies or other biomolecules.

Features

⦁ Label 5-20 ug or 20-50 ug VHH with single exposed cysteine residue

⦁ Choice of 7 CF® Dye colors

⦁ Simple 2 hour labeling, no purification

⦁ No purification, 100% recovery

Kit components

⦁ Lyophilized Reactive CF® Dye

⦁ Mix-n-Stain™ Reaction Buffer, 5X

⦁ Mix-n-Stain™ Activating Agent

⦁ Mix-n-Stain™ Quenching Buffer, 10X

⦁ Mix-n-Stain™ Storage Buffer, 4X

Superior CF® Dyes

These kits feature a choice of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to other commercially available fluorescent dyes. Learn more about CF® Dyes.

Choosing the Right Kit for Your Application

Before selecting a Mix-n-Stain™ Nanobody Thiol Labeling Kit, please download the Product Protocol to see our Kit Compatibility guidelines.

For labeling IgG antibodies, please use one of our Mix-n-Stain™ Antibody Labeling Kits. We also offer Mix-n-Stain™ Small Ligand Labeling Kits, for labeling SNAP-tag®, CLIP-tag™, and HaloTag® ligands, or other small molecules. See Mix-n-Stain™ Product Lines below for our full selection of antibody, protein, and ligand labeling kits.

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