Product Name:Gelsolin (GS), ELISA Kit 

Full Product Name:Human Gelsolin (GS) ELISA Kit

Product Gene Name:GS elisa kit

Research Use Only:For Research Use Only. Not for use in diagnostic procedures.

Molecular Weight:80,929 Da

Species Reactivity:Human

Specificity:No cross-reaction with other factors.

Samples:Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant GS concentration

Assay Type:Sandwich

Detection Range:100 ng/ml-1.56 ng/ml

Sensitivity:0.5 ng/ml.

Intra-assay Precision:<= 8%

Inter-assay:<= 12%

Preparation and Storage:Store all reagents at 2-8 degree C.

ISO Certification:Manufactured in an ISO 9001:2015 Certified Laboratory.

Product Note:Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Other Notes: Small volumes of GS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GS purchase:

MBS264815 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Gelsolin (GS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GS. The ELISA analytical biochemical technique of the MBS264815 kit is based on GS antibody-GS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Related Product Information for

GS elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI GI #:51854227

NCBI GeneID:296654

NCBI Accession #:NP_001004080.1

NCBI GenBank Nucleotide #:NM_001004080.1

UniProt Related Accession #:Q68FP1

NCBI Official Full Name:gelsolin

NCBI Official Synonym Full Names:gelsolin

NCBI Official Symbol:Gsn  

NCBI Protein Information:gelsolin; ADF; brevin; actin-depolymerizing factor

UniProt Protein Name:Gelsolin

UniProt Synonym Protein Names:Actin-depolymerizing factor; ADF; Brevin

UniProt Gene Name:Gsn  

UniProt Synonym Gene Names:ADF  

UniProt Entry Name:GELS_RAT

NCBI Summary for GS:plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; may regulate sperm release and turnover of the blood-testis barrier [RGD, Feb 2006]

UniProt Comments for GS

Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Nuclear receptor co-regulator

Cellular Component: ruffle; extracellular space; focal adhesion; protein complex; perinuclear region of cytoplasm; lamellipodium; extracellular region; cytosol; actin cytoskeleton

Molecular Function: calcium ion binding; actin binding

Biological Process: regulation of cell adhesion; actin filament severing; phosphoinositide-mediated signaling; wound healing; apoptosis; actin nucleation; vesicle-mediated transport; response to ethanol; actin filament polymerization; tissue regeneration; actin polymerization and/or depolymerization; response to folic acid; oligodendrocyte development; barbed-end actin filament capping; aging

Research Articles on GS

1. It was concluded that cytoskeletal effects of Cd in mesangial cells are partially mediated by Cd-dependent activation of CaMK-IIdelta, binding of CaMK-IIdelta and gelsolin to actin filaments, and cleavage of gelsolin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

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