Immortalized Human Brain Microvascular Endothelial Cells-GFP

Cat.No.: CSC-C12026Z

Species: homo sapiens

Morphology: Polygonal

Culture Properties: Adherent

Cat.No.CSC-C12026Z

DescriptionImmortalized Human Brain Microvascular Endothelial Cells-GFP provided by Creative Bioarray have been developed by immortalizing primary human brain endothelial cells with SV40 Large T antigen and transfected with tGFP.

Specieshomo sapiens

Recommended MediumSuperCult® Immortalized Human Brain Microvascular Endothelial Cell Medium (Cat No.: CM-C12026Z)

Freezing MediumComplete medium supplemented with 10% (v/v) DMSO

Culture PropertiesAdherent

MorphologyPolygonal

Immortalization MethodSV40 large T antigen

ApplicationFor Research Use Only

Growth PropertiesCells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.

ShippingDry Ice.

Quality ControlReal Time PCR was used to quantify SV40T gene expression in immortalized cell line.

Free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations.

Storage and ShippingDirectly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20°C.

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