Human Chondrocytes: Re-Differentiated: HC-RD

Description

⦁ Human Chondrocytes Re-Differentiated (HC-RD) have been encapsulated in an alginate bead suspension.  HC-RD exhibit spherical morphology and have regained their ability to produce the phenotypic markers Aggrecan and Type II Collagen.  RD-HC can be used in tissue engineering experiments aimed to repair cartilage defects and to study chondrocyte differentiation and the effects of Growth factors & Cytokines on the suppression and maintenance of differentiated cartilage phenotype.

⦁ Instructions to release HC-RD from Alginate Beads

⦁ Remove Chondrocyte Differentiation Medium

⦁ Wash the beads 2 times with 0.155 M NaCl

⦁ Add 3 volume of Depolymerization Solution per volume of packed alginate beads.

⦁ Mix at room temperature for 30 minutes with gentle agitation until the beads have completely dissolved

⦁ Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes

⦁ Wash the cells 2 times with 0.155 M NaCl

⦁ Media Salts & Chemicals used to Release HC-RD from alginate beads:

⦁ Calcium Chloride Solution: Formulated to induce polymerization of Sodium Alginate.

⦁ Sodium Alginate:  Encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes

⦁ Depolymerization Solution (DS): Consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.

Details

Tissue:Normal healthy human articular cartilage

QC:No bacteria, yeast, fungi, mycoplasma, virus

Cryovial:N/A

Kit:N/A

Proliferating:Shipped in Differentiation Medium (flasks)

Doublings:N/A

Note:Cells shipped 10 days after encapsulation.  Re-Differentiated Chondrocytes expected 3 weeks after encapsulation, retain phenotype at least 1 week after complete re-differentiation.

Applications:Laboratory research use only (RUO). Not for human, clinical, diagnostic or veterinary use.

 Chondrocytes cultured in monolayer quickly lose their phenotypic characteristics and no longer express Collagen type II and sulfated proteoglycan. Encapsulation of de-differentiated chondrocytes for 3 to 4 weeks in alginate beads will induce re-differentiation and ECM production in chondrocytes.

⦁ Calcium Chloride Solution is formulated to induce polymerization of Sodium Alginate.

⦁ Chondrogenesis Kit contains all media & solutions for the encapsulization of chondrocytes in alginate beads

⦁ Sodium Alginate encapsulates chondrocytes to maintain their phenotypic characterization after isolation or to induce cultured chondrocytes to re-differentiate to chondrocytes.

⦁ Depolymerization Solution (DS) consists of sodium citrate, and dissolves alginate beads to release re-differentiated chondrocytes.

Instructions for Releasing Re-differentiated Chondrocytes from Alginate Beads

1. Remove Chondrocyte Differentiation Medium

2. Wash the beads 2 times with 0.155 M NaCl

3. Add 3 volume of Depolymerization Solution per volume of packed alginate beads

4. Mix at room temperature for 30 minutes

5. Apply gentle agitation until the beads have completely dissolved

6. Pellet re-differentiated chondrocytes at 1000 rpm for 10 minutes

7. Wash the cells 2 times with 0.155 M NaCl

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