CK-BB ELISA kit (Mouse) (OKCD07926)

Product Info

Predicted Species Reactivity:Mouse|Mus musculus

Application:ELISA-Sandwich

ELISA Kit Detection Method:Colorimetric

ELISA Kit Duration:3h

ELISA Kit Principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase B (CK-BB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Creatine Kinase B (CK-BB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Creatine Kinase B (CK-BB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Creatine Kinase B (CK-BB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ELISA Kit Range:78-5,000pg/mL

ELISA Kit Reproducibility:Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Creatine Kinase B (CK-BB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Creatine Kinase B (CK-BB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Reconstitution and Storage:-20°C|2°C to 8°C

Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Sensitivity:< 30pg/mL

Gene Symbol:Ckb

Gene Full Name:creatine kinase, brain

Alias Symbols:B-CK, Bck, Ck-3, Ck3, Ckbb, CPK-B, creatine kinase B chain, creatine kinase B-type, creatine phosphokinase B-type.

NCBI Gene Id:12709

Protein Name:Creatine kinase B-type

Description of Target:Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Uniprot ID:Q04447

Protein Accession #:NP_067248.1

Nucleotide Accession #:NM_021273.4

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