anti-CRYAA antibody :Mouse anti-Human A crystallin A Monoclonal Antibody

Product Name:A crystallin A (CRYAA), Monoclonal Antibody 

Full Product Name:A crystallin A antibody

Product Synonym Names:Heat shock protein beta-4; HspB4; Alpha-crystallin A chain; 

Heat shock protein beta 4; Acry-1; Alpha crystallin A chain; CRYA 1; CRYA1; CRYAA; 

Crystallin Alpha 1; Crystallin alpha A; HSPB 4; Zonular Central Nuclear Cataract

Product Gene Name:anti-CRYAA antibody[Similar Products]

Research Use Only:For Research Use Only. Not for use in diagnostic procedures.

OMIM:U05569 mRNA

3D Structure:ModBase 3D Structure for P02489

Clonality:Monoclonal

Isotype:IgG1, k

Clone Number:c9F2

Host:Mouse

Species Reactivity:Human

Purity/Purification:By protein-G affinity chromatography

Form/Format:Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.

Concentration:1mg/ml (determined by BCA assay) (lot specific)

Immunogen:Recombinant human Crystallin alpha A (1-173aa) purified from E. coli

Preparation and Storage

1. Can be stored at 2°C to 8°C for 1 week.

2. For long term storage, aliquot and store at -20°C to -80°C.

3. Avoid repeated freezing and thawing cycles.

Other Notes

Small volumes of anti-CRYAA antibody vial(s) may occasionally become entrapped in

the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the

vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products 

may require to ship with dry ice and additional dry ice fee may apply.

Related Product Information for anti-CRYAA antibody

alpha-crystallins, a major protein of the ocular lens, are thought to play a role in maintaining lens transparency, which are 

composed of two gene products alpha-A and alpha-B, for acidic and basic, respectively. alpha-crystallins can be induced 

by heat shock and are members of the small heat shock protein (sHSP). They act as molecular chaperones and hold unfolded 

or misfolded proteins in large, water-soluble low molecular weight aggregates. These heterogeneous aggregates consist of

 30-40 subunits of the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of alpha-crystallins 

are an autokinase activity and the participation in the intracellular architecture.Product Categories/Family for anti-CRYAA antibody,

Heat Shock Proteins,Applications Tested/Suitable for anti-CRYAA antibody,ELISA (EIA), Western Blot (WB), FACS 

Application Notes for anti-CRYAA antibody.Since applications varies, however, each investigation 

should be titrated by reagent to obtain optimal results.

Western Blot (WB) of anti-CRYAA antibody.The recombinant proteins were resolved by SDS-PAGE,

 transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:1000).

Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

This antibody is not shown cross-activity about Crystallin alpha B.

Lane 1.: Recombinant human Crystallin alpha A protein

Lane 2.: Recombinant human Crystallin alpha B protein

anti-CRYAA antibody Western Blot (WB) (WB) image

Western Blot (WB) of anti-CRYAA antibody

The cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed 

with anti-human Crystallin alpha A antibody (1:3000).Proteins were visualized using a goat 

anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1.: 293T cell lysate

Lane 2.: Crystallin alpha A Transfected 293T cell lysate

anti-CRYAA antibody Western Blot (WB) (WB) image

Flow Cytommetry (FC) of anti-CRYAA antibody

Flow cytometry analysis of Crystallin alpha A in Balb/3T3 cells. The cell was stained with MBS200007 at 2-5ug for 1x10^6cells (red). 

A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), 

cells without incubation with primary and secondary antibody was used as the negative control (light gray).

anti-CRYAA antibody Flow Cytommetry (FC) image

NCBI/Uniprot data below describe general gene information for CRYAA. It may not necessarily be applicable to this product.

NCBI GI #:4503055

NCBI GeneID:1409

NCBI Accession #:NP_000385

NCBI GenBank Nucleotide #:NM_000394.3

UniProt Primary Accession #:P02489

UniProt Secondary Accession #:Q53X53

UniProt Related Accession #:P02489

NCBI Official Full Name:alpha-crystallin A chain

NCBI Official Synonym Full Names:crystallin alpha A

NCBI Official Symbol:CRYAA  

NCBI Official Synonym Symbols:CRYA1; HSPB4; CTRCT9  

NCBI Protein Information:alpha-crystallin A chain

UniProt Protein Name:Alpha-crystallin A chain

UniProt Synonym Protein Names:Heat shock protein beta-4; HspB4

Protein Family:Alpha-crystallin

UniProt Gene Name:CRYAA  

UniProt Synonym Gene Names:CRYA1; HSPB4; HspB4 

NCBI Summary for CRYAA

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: 

alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the 

small heat shock protein (HSP20) family.They act as molecular chaperones although they do not renature proteins and release

 them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications 

decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits 

have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the 

intracellular architecture. The encoded protein has been identified as a moonlighting proteinbased on its ability to perform 

mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed;alpha-A is preferentially 

restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal 

dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014]

UniProt Comments for CRYAA

Contributes to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation 

of various proteins under a wide range of stress conditions.

Product References and Citations for anti-CRYAA antibody

Fujii N., et al., (2003) Mol. Vis. 9: 915-22; Laksanalamai P., et al., (2004) Extremophiles 8: 1-11

Research Articles on CRYAA

1. The results suggest that the N-terminal domain of CRYAA is required during in vitro complex formation with filensin and phakinin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics,

 prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you

 will properly test and use any Products purchased from MyBioSource in accordance with industry standards. 

MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably

believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not 

be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet

 at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource 

within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.