anti-EBV/LMP-1 antibody :: Rabbit EBV/LMP-1 Monoclonal Antibody

Catalog # MBS684137

Product Name:EBV/LMP-1, Monoclonal Antibody 

Full Product Name:Anti - EBV/LMP-1

Product Gene Name:anti-EBV/LMP-1 antibody

Research Use Only:For Research Use Only. Not for use in diagnostic procedures.

Chromosome Location:NC_007605.1 (166461..169055, complement)

3D Structure:ModBase 3D Structure for P03230

Clonality:Monoclonal

Clone Number:D24-G

Host:Rabbit

Specificity:Human

Form/Format:20 mM Tris-HCI, pH 8.0

Immunogen:Peptide derived from the internal region of Epstein-Barr virus (EBV), Latent Membrane Protein-1 (LMP1). Antibody recognizes the epitope between Asp293 -Asp312

Stabilizer:20 mg/ml BSA

Preservative:0.05% NaN3

Cellular localization:Nucleus

Positive Control:Lymph node Hodgkins lymphoma tissue

PRECAUTIONS

1. We strongly recommend to use Primary Antibody Diluent eventually alternative diluent containing protease free BSA at the concentrations > 1mg/ml) for dilution of concentrated antibodies, otherwise the warranty might be voided.

2. Centrifuge the vial before use.

3. Intended for professional use in laboratories. .

4.Do not use after expiration date stamped on vial label. .

5. Avoid contamination of the reagent. .

6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results. .

7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous. .

8. Disposal of waste material must be conducted in accordance with local regulations. .

9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.

Preparation and Storage:Store at +4°C.

ISO Certification:Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Other Notes

Small volumes of anti-EBV/LMP-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable for anti-EBV/LMP-1 antibody

Immunohistochemistry (IHC) - Formalin/Paraffin

Application Notes for anti-EBV/LMP-1 antibody

IHC-P: 1:100

IHC-P PROTOCOL:

1. Deparaffinize the section in 3 changes of xylene, 5 minutes each.

2. Wash the section in 96%, 80% and 0% thyl alcohol for 10 minutes each.

3. Rinse in distilled water.

4. Block the endogenous peroxidase by incubating the tissuein 3% hydrogen peroxide (H2O2) for 10 minutes.

5. Wash in distilled water for 5 minutes.

6. For antigen retrieval: immerse the slide in the citrate buffer, pH 6.0, 0.05% Tween-20*, and incubate in ater bath at 96°C for 40 minutes. (Alternatively adjust to your own protocol, keeping the required pH) .

7. Remove the staining to room temperature and let the slide to cool (in citrate buffer, pH 6.0) for 20 minutes.

8. Rinse in distilled water.

9. Wash in 0.05 M Tris-HCl, pH 7.6 buffer supplemented with 0.2% of Tween-20 (buffer A) for 5 minutes.

10. CONCENTRATED: Incubate the section with primary antibody at the dilution 1:100 -1:200 for 1 hour in the closed wet chamber.

READY TO USE (RTU): Incubate the section with primary antibody (ready to use)for 1 hour in a closed wet chamber.

11. Wash twice 5 minutes with buffer A.

12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP -Peroxide -DAB). Micropolymer-HRP detection kit rabbit/mouse .

13. Wash twice 5 minutes with buffer A.

14. Apply the chromogen (DAB), 1 -3 minutes.

15. Wash in water for 10 minutes.

16. Stain in hematoxylin for 5 minutes.

17. Wash in water for 10 minutes.

18. Dehydrate the section in 2 changes of 96% ethyl alcohol for 5 minutes each.

19. Wash the section in 2 changes of xylene for 2 minutes each.

20. Mount the slide for observation.

Citrate Buffer(10mM Citric Acid, 0.05% Tween-20, pH 6.0):

Citric acid (anhydrous) ----------1.92 g; Distilled water ---------1000 ml.

Mix to dissolve in 700 ml of distilled water. Adjust pH to 6.0 with 1M NaOH and then add 0.5 ml of Tween-20 and mix well.

Adjust the final volume to 1 liter with distilled water.

Store this solution at room temperature for 3 months or at +4°C for longer storage.

VENTANA PROTOCOL:

PROCEDURE: U ultraView DAB

1. Deparafinization.

2. Heating (72 °C) at the medium temperatures. Deparafinization.

3. Cell conditioning.

4. ULTRA conditioner #1.

5. Heating glass (95 °C), incubation 8 min. (Cell conditioner #1; buffer CC1).

6. ULTRA CC1 solution application â 36 min.

7. Antibody incubation temperature.

8. Heating glass (36 °C), incubation 4 min.

9. Titration.

10. Hand apply â primary antibody 100 ?l. Incubation 32 min.

11. ultraWash.

12. Nuclear stain.

13. Hematoxylin II application â one drop (nuclear stain). Cover and incubate 12 min.

14. After nuclear stain.

15. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min.

LEICA BOND MAX PROTOCOL:

Protocol F:

• Visualization system: BOND Refine DS9800

• Epitope retrieval / heating time / temperature: ER2 / 30 min. / 100 °C

• Incubation of primary antibody / temperature: 30 min. / 20 °C

NCBI GI #:126373

NCBI GeneID:3783750

NCBI Accession #:P03230.1

UniProt Primary Accession #:P03230

UniProt Secondary Accession #:Q777A4

UniProt Related Accession #:1011284A

NCBI Official Full Name:Latent membrane protein 1

NCBI Official Symbol:LMP1  

NCBI Protein Information:LMP1; hypothetical protein

UniProt Protein Name:Latent membrane protein 1

UniProt Synonym Protein Names:Protein p63

UniProt Gene Name:LMP1  UniProt Synonym Gene Names

LMP-UniProt Entry Name:LMP1_EBVB9

UniProt Comments for EBV/LMP-1

Function: Acts as a CD40 functional homolog to prevent apoptosis of infected B-lymphocytes and drive their proliferation. Functions as a constitutively active tumor necrosis factor receptor that induces the activation of several signaling pathways, including those of the nuclear factor-kappa-B family. LMP1 signaling leads to up-regulation of antiapoptotic proteins and provide growth signals in latently infected cells. It is a short-lived protein probably degraded by the proteasome.

Subunit structure: Interacts (via PXQXT motif) with host tumor necrosis factor receptor-associated factor (TRAF) proteins TRAF1, TRAF2, TRAF3 and TRAF5.

Subcellular location: Host cell membrane; Multi-pass membrane protein Ref.8.

Domain: Two regions, C-terminal-activating region 1 (CTAR1) and CTAR2, have been identified within the cytoplasmic carboxy terminal domain that activates NF-kappa-B. Ref.7

Post-translational modification: Ubiquitinated on the N-terminus. Ref.8

Sequence similarities: Belongs to the herpesviridae LMP-1 family.

Research Articles on EBV/LMP-1

1. Results indicate an important role for LMP1 and Cabin1 in regulating apoptosis in nasopharyngeal carcinoma cells in response to genotoxic stress.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

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