Murine Anti- Factor VIII

GMA-8021

Clone GMA-8021

Background

Factor VIII (FVIII) is a heterodimer consisting of a heavy chain (ranging in mass from 90 to 200 kDa) bound via metal ions to a light chain (80 kDa). In plasma, FVIII circulates in an inactive form bound to von Willebrand factor. Following activation by factor Xa or thrombin, factor VIIIa can function as cofactor for the enzyme factor IXa in the activation of factor X in the presence of phospholipid and Ca2+ . Absent or defective FVIII is the cause of the X-linked recessive bleeding disorder hemophilia A. GMA- 8021 (also known as 2-76 or 76-1B) recognizes the A2 domain of FVIII, is strongly inhibitory 1 , and suitable for ELISA and bio-layer interferometry applications.

Description

Species Reactivity:Human

Source:Murine

Specificity:A2 domain

Immunogen:B-domain deleted recombinant Human Factor VIII

Isotype:IgG2a

Application

ELISA:Binds the A2 domain of recombinant human factor VIII in solid-phase ELISA.

Immunoblotting:Not recommended.

Inhibition:Strongly inhibitory in aPTT clotting assay.1

Antibody References:

1.R.C. Markovitz et al. (2013). Blood. 121(14):2785-2795.

2.J. Gewirtz et al. (2008). J Thromb Haemost. 6(7):1160-1166.

3.R.B. Lyde et al. Blood Advances. (2019). 3(9):1368-1378.

4.G. D. Minno et al. (2014). Haemophilia. 20 (Suppl. 6):27–43.

5.G.E. Gilbert et al. (2012). Blood. 120(9):1923–1932.

6.V.A. Novakovic et al. (2011). Biochem J. 435(1):187–196.

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