SPAM1 ELISA Kit (Human) (OKCA00696)

Size:96 Wells

Product Info:Predicted Species Reactivity Human

ELISA Kit Detection Method:Colorimetric, OD450 nm

ELISA Kit Duration:~ 3.5 Hours

ELISA Kit Principle:Aviva Systems Biology SPAM1 ELISA Kit (Human) (OKCA00696) is based on standard sandwich enzyme-linked 

immuno-sorbent assay technology. An antibody specific for SPAM1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). 

Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SPAM1 

is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate 

is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating 

a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance 

at 450 nm and is quantitatively proportional to the amount of sample SPAM1 captured in the well.

ELISA Kit Range:15.6 pg/mL-1,000 pg/mL

ELISA Kit Reproducibility

Intra-assay Precision: 3 samples with known low, middle and high level SPAM1 were tested with 20 replicates across one plate.

Inter-assay Precision: 3 samples with known low, middle and high level SPAM1 were tested on three different plates with 8 replicates, respectively.

Intra-Assay CV: <8%

Inter-Assay CV: <10%

Reconstitution and Storage:Store as indicated in product manual.

Sample Type:Serum, Plasma, Urine, Tissue Homogenates

Sensitivity:3.9 pg/mL

Assay Info:Assay Methodology: Quantitative Sandwich ELISA

Gene Symbol:SPAM1

Gene Full Name:Sperm adhesion molecule 1

Alias Symbols:HYA1, PH20, HYAL1, HYAL3, HYAL5, PH-20, SPAG15, HEL-S-96n

NCBI Gene Id:6677

Protein Name;Hyaluronidase PH-20

Description of Target:Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that 

surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid 

which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Uniprot ID:P38567

Protein Accession #:NP_001167515.1

Nucleotide Accession:#NM_001174044.1

견적 문의: aibiohub@aibiohub.co.kr  홈페이지: www.aibiohub.co.kr 

견적 문의: https://aibiohub.co.kr/estimate/write