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[공식 대리점] Pronetbio-Yeast One Hybrid (TF-Centered) Vector Kit,효모 원-하이브리드 (TF 중심) 벡터 키트( RY8012)
- AI바이오허브 오래 전 2025.01.29 00:11 Bulletin Board 인기
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Yeast One Hybrid (TF-Centered) Vector Kit
CAT: RY8012
Product Overview
The TF-Centered Yeast One-Hybrid (Y1H) Vector Kit is designed to identify transcription factor (TF) recognition elements efficiently and accurately. Based on the principle of GAL4 AD-mediated activation of the reporter gene HIS3, this system enables researchers to uncover interactions between proteins and DNA by facilitating growth on 3-AT-containing media.
This kit includes a randomized 7-bp DNA motif library containing 3×4⁷ sequences, enabling robust identification of transcription factor binding sites. It supports high-throughput and reliable experiments, offering flexibility across diverse research scenarios in transcription factor-DNA interaction studies.
Key Features
Comprehensive Coverage: The randomized DNA motif library contains all possible 7-bp sequences, ensuring compatibility with various transcription factors and species.
High Efficiency: Accurately identifies TF recognition elements in a fast and straightforward workflow.
Reliable System: Includes positive controls (pHIS2-p53 + pGAD53m) and precise plasmid and primer combinations for reproducibility.
Flexible Application: Ideal for both basic and applied research in transcriptional regulation.
Product Components
Component | Name | Specification | Volume | Quantity |
Plasmid | pGADT7 | 100 ng/μL | 20 μL | 1 tube |
Plasmid | pHIS2 | 100 ng/μL | 20 μL | 1 tube |
Plasmid | pHIS2-p53 | 100 ng/μL | 20 μL | 1 tube |
Plasmid | pGAD53m | 100 ng/μL | 20 μL | 1 tube |
Strain | Promoter Library Yeast | Suspension | 1 mL | 4 tube |
Strain | Y187 | Inoculated Plate | 1 plate | 1 plate |
Primer | pHIS2-F | Lyophilized powder | 2 OD | 1 tube |
Primer | pHIS2-R | Lyophilized powder | 2 OD | 1 tube |
Primer | AD-R | Lyophilized powder | 2 OD | 1 tube |
Primer | T7-F | Lyophilized powder | 2 OD | 1 tube |
Experimental Guidelines
Storage and Shipping
Item | Storage Condition | Shipping Condition |
|---|---|---|
Plasmids | -20°C | Room Temperature |
Strains | Room Temperature | Room Temperature |
Primers | -20°C | Room Temperature |
Precautions
1. To ensure strain viability, streak the strain onto a YPDA agar plate before liquid culturing.
2. Store strains at -80°C with 50% glycerol to prevent repeated freeze-thaw cycles.
3. This product is for research use only and not for clinical, diagnostic, or therapeutic applications.
4. Maintain sterile conditions to avoid contamination.
FAQs
Why is self-activation testing necessary?
Self-activation ensures the reporter gene is not expressed due to endogenous transcription factors or promoter leakage, providing reliable results.
Can this kit be reused?
Yes, the plasmids and yeast strains in the kit can be expanded and preserved for repeated use.
Why is this system preferred over other Y1H systems?
Unlike EGY48-LacZ systems, this kit does not require additional staining reagents, making it simpler and faster to use.
Self-prepared Materials
1. Sterile PCR tubes, tips, centrifuge tubes, shakers, incubators, and reagents like 50% PEG3350, 1M LiAc, X-α-Gal, and 3-AT.
2. (Optional from Pronetbio) Yeast media (YPDA, SD-Trp, SD-Trp/-Leu/-His), yeast plasmid extraction kits, and positive clone amplification kits.
