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[공식 대리점] Pronetbio-Yeast Two-Hybrid (Membrane System) Vector Kit,효모 이중 하이브리드 (막 시스템) 벡터 키트, RY8010
- AI바이오허브 오래 전 2025.01.28 18:07 Bulletin Board 인기
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Yeast Two-Hybrid (Membrane System) Vector Kit
CAT: RY8010
Product Overview
The Yeast Two-Hybrid (Membrane System) Vector Kit provides a comprehensive solution for membrane protein library screening and one-to-one verification of protein interactions. It includes the NMY51 yeast strain (MATa type), three Bait plasmids (pBT3-STE, pBT3-SUC, and pBT3-N), and the Prey plasmid pPR3-N. Positive control plasmid combinations (pBT3-STE-OsPT2 + pPR3-N-OsPP95) and a negative control (Bait plasmid + pPR3-N) are also included to ensure reliable results.
Key Features
High versatility: Supports both membrane protein library screening and one-to-one interaction analysis.
Positive and negative controls included for validation.
Broad applicability across various membrane and cytoplasmic protein studies.
Product Components
Component | Name | Concentration | Specification | Quantity |
Plasmids | pPR3-N | 100ng/uL | 20uL | 1 tube |
pBT3-STE | 100ng/uL | 20uL | 1 tube | |
pBT3-SUC | 100ng/uL | 20uL | 1 tube | |
pBT3-N | 100ng/uL | 20uL | 1 tube | |
pBT3-C | 100ng/uL | 20uL | 1 tube | |
pBT3-STE-OsPT2 | 100ng/uL | 20uL | 1 tube | |
pPR3-N-OsPP95 | 100ng/uL | 20uL | 1 tube | |
pPR3-N-OsPP95CT | 100ng/uL | 20uL | 1 tube | |
Strain | NMY51 | Inoculated Plate | Diameter:90mm | 1 plate |
Primers | pPR3-N-F | Lyophilized powder | 2OD | 1 tube |
pPR3-N-R | Lyophilized powder | 2OD | 1 tube | |
pBT3-SUC/STE-F | Lyophilized powder | 2OD | 1 tube | |
pBT3-SUC/STE-R | Lyophilized powder | 2OD | 1 tube | |
pBT3-C-F | Lyophilized powder | 2OD | 1 tube | |
pBT3-C-R | Lyophilized powder | 2OD | 1 tube | |
pBT3-N-F | Lyophilized powder | 2OD | 1 tube | |
pBT3-N-R | Lyophilized powder | 2OD | 1 tube |
Primer Sequence
Primer Name | Sequence | Applicable Vector |
pPR3-N-F | CGGTAAAACCGGAACATTGGA | pPR3-N |
pPR3-N-R | ACTTCAGGTTGTCTAACTCCT | |
pBT3-SUC/STE-F | TGGCATGCATGTGCTCTG | pBT3-SUC |
pBT3-SUC/STE-R | GTAAGGTGGACTCCTTCT | pBT3-STE |
pBT3-N-F | CAGAAGGAGTCCACCTTAC | pBT3-N |
pBT3-N-R | AAGCGTGACATAACTAATTAC | |
pBT3-C-F | TATAAAACTCTTGTTTTCTTC | pBT3-C |
pBT3-C-R | GGTGGACTCCTTCTGAATGTT |
Storage and Shipping Conditions
Plasmids: Store at -20°C; shipped at room temperature.
Strains: Store and ship at room temperature.
Primers: Store at -20°C; shipped at room temperature.
Experimental Guidelines
Selection of bait plasmid vectors:
Type | N-terminal | C-terminal | Bait Vector | Prey Vector |
1 | inside | outside | pBT3-N | pBT3-N |
2 | outside | inside | pBT3-STE | pBT3-N |
3 | inside | inside | pBT3-N or pBT3-STE | pBT3-N |
4 | No transmembrane structure | pBT3-SUC | pBT3-N | |
