High-Performance Yeast Colony PCR Kit for Rapid and Reliable Amplification

ProNet Biotech's Second-Generation Yeast Colony PCR Kit offers a fast, reliable, and high-throughput solution for amplifying target sequences from yeast colonies. Powered by our enhanced Yeast Buster Pro lysis buffer and green-dye-loaded PCR mix, this kit ensures superior lysis efficiency, high product stability, and excellent compatibility with downstream applications like electrophoresis and TA cloning.

Product Highlights

· Rapid Lysis with Yeast Buster Pro: Efficiently breaks yeast cell walls and preserves DNA integrity.

· Ready-to-Load PCR Mix: Includes green dye for direct gel electrophoresis post-amplification.

· Freeze-Thaw Stable: Contains stabilizers to ensure performance consistency.

· TA-Cloning Ready: PCR products have 3' A-overhangs, suitable for TA cloning.

· Compatible with All Yeast Strains: Optimized for Saccharomyces cerevisiae and other yeast types.

Application Scope

· Suitable for all types of yeast strains

· Designed for rapid amplification of recombinant plasmid target sequences directly from yeast colonies

Kit Components

Workflow Overview

From Colony to Gel in 3 Simple Steps

· Yeast Cell Lysis: Use Yeast Buster Pro to disrupt cells at 100°C for 5 min.

· PCR Reaction: Amplify target gene with optimized primers and ready-to-use 2× PCR mix.

· Gel Electrophoresis: Load PCR product directly onto gel for analysis.

Experimental Protocol Summary

Lysis Reaction Setup:

Add 5 μl Yeast Buster Pro to PCR tube

Pick colony and suspend in lysis buffer

Heat at 100°C for 5 min

PCR Reaction Setup (Total volume: 50 μl):

25 μl 2× Yeast PCR Mix

2 μl Forward Primer (10 μM)

2 μl Reverse Primer (10 μM)

1 μl lysate as template

Add ddH₂O to 50 μl

Thermal Cycler Conditions:

Initial Denaturation: 95°C, 3 min

30–32 cycles of:

Denaturation: 95°C, 15 sec

Annealing: Tm − 5°C, 15 sec

Extension: 72°C, 15 sec/kb

Final Extension: 72°C, 5 min

Hold: 4°C

Common Troubleshooting Tips

Ordering Information

Catalog Number: RY8031
Specifications: 40T / 80T / 160T / 320T
Storage: -20°C (avoid repeated freeze-thaw cycles)
Shelf Life: 12 months

Disclaimer: This product is intended solely for research purposes. It is not intended for human or veterinary diagnostic or therapeutic use.

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