Advanced Yeast One-Hybrid System for DNA-Protein Interaction Studies

The Yeast One Hybrid (Gene-Centered) Vector Kit is designed for researchers studying DNA-protein interactions to explore gene expression regulation, identify DNA binding sites, and analyze DNA-binding domains. Developed from the Yeast Two-Hybrid system, this kit offers a reliable, streamlined approach to uncover key interactions in eukaryotic cells.

CAT：RY8009

Price and Instruction: Available upon inquiry

Product Overview

This kit includes the Y187 strain (MATα type) and essential plasmids pHIS2 and pGADT7. The yeast one-hybrid assay leverages GAL4 AD to activate HIS3 gene expression when prey proteins bind to the bait DNA sequence. Growth on 3-AT supplemented media confirms DNA-protein interaction. The included primers, plasmids, and controls facilitate amplification, sequencing, and experimental validation.

Key Features:

Positive Control: pHIS2-p53 + pGAD53m;

Selection Markers: Trp for pHIS2, Leu for pGADT7;

Includes high-quality primers: pHIS2-F, pHIS2-R, T7-F, AD-R.

Product Components

Storage and Shipping Conditions

Plasmids: Store at -20°C; shipped at room temperature.

Strains: Store and ship at room temperature.

Primers: Store at -20°C; shipped at room temperature.

Experimental Guidelines

Precautions

1. To ensure strain viability, streak the strain onto a YPDA agar plate before liquid culturing.

2. Store strains at -80°C with 50% glycerol to prevent repeated freeze-thaw cycles.

3. This product is for research use only and not for clinical, diagnostic, or therapeutic applications.

4. Maintain sterile conditions to avoid contamination.

FAQs

1. Why is self-activation testing required?

Self-activation is a common phenomenon where the promoter induces background expression of the reporter gene. Testing ensures that the bait DNA does not produce false positives.

2. Can non-transcription factor proteins be identified?

Yes. Yeast one-hybrid assays detect all proteins binding to the bait DNA, including non-transcription factors and potential false positives.

3. Why doesn't the Y187-pHIS2 system require staining reagents?

Unlike the EGY48-LacZ system, Y187-pHIS2 confirms interactions through HIS3 expression on selective media.

4. Is the mating method applicable for Y1H?

No. Both Y1HGold and Y187 strains are MATα type, and mating requires MATa and MATα strain compatibility.

Self-prepared Materials

1. Sterile PCR tubes, tips, centrifuge tubes, shakers, incubators, and reagents like 50% PEG3350, 1M LiAc, X-α-Gal, and 3-AT.

2. (Optional from Pronetbio) Yeast media (YPDA, SD-Trp, SD-Trp/-Leu/-His), yeast plasmid extraction kits, and positive clone amplification kits.

Ready to Advance Your Research?

Contact us today for a detailed consultation and product quotation. Let us help you unlock new insights in DNA-protein interactions research. When inquiring about this product, please reference Product Code: RY8009.

Statement: All products are for research purposes only. Not for use in diagnostic or therapeutic applications.

Related Service: Yeast One-Hybrid (Y1H) Screening Service (Service Code: SRY8009) 

Keywords: Yeast One-Hybrid Kit, DNA-Protein Interaction Analysis, Gene Expression Regulation Study, pHIS2 and pGADT7 Plasmids, Y187 Yeast Strain.