High-Purity Plant DNA Extraction Kit | PCR-Ready Genomic DNA in 1 Hour

Product Overview

This kit is designed for efficient isolation of high-quality genomic DNA from plant tissues rich in polysaccharides and polyphenols, such as cotton, pine, ginkgo, banana, grape, wheat, peanut, and various fungi. It employs PR DNA Columns and an optimized buffer system that enables rapid and reliable purification. The extracted DNA shows high purity and is compatible with downstream applications such as PCR, restriction digestion, and hybridization.

· Complete purification within 1 hour

· Compatible with fresh or dried plant tissues

· High yield and purity with minimal contaminants

· No need for phenol or chloroform extraction

Kit Components

Note: Binding Buffer BD and Wash Buffer W* require the addition of anhydrous ethanol before use.*

Shipping and Storage

· RNase A: Shipped with ice packs; store at -20°C;

· Columns and buffers: Shipped and stored at room temperature;

· Shelf life: 12 months.

Protocol Highlights

· Preheat lysis buffer LB and prepare extraction buffer with β-mercaptoethanol

· Homogenize plant tissue in liquid nitrogen

· Lyse, centrifuge, and bind DNA to the column

· Wash with ethanol-containing buffers

· Elute with preheated buffer to improve yield

· Typical DNA extraction takes less than 1 hour

Precautions

· Avoid prolonged exposure of buffers to air to prevent pH changes and degradation

· Buffers may precipitate at low temperatures; warm to 65°C to dissolve

· Binding Buffer and Protein Removal buffers contain irritants; use personal protective equipment

Frequently Asked Questions (FAQs)

Q: No visible PCR bands after extraction?
A: This may be due to low DNA yield, degradation, or impurities. Ensure complete lysis and use fresh samples.

Q: Low yield?
A: Possible causes include insufficient sample amount, poor grinding, or ineffective elution. Try preheating the elution buffer or repeating the elution step.

Q: DNA degradation?
A: Degradation can result from repeated freeze-thaw cycles or harsh handling. Use fresh materials, keep cold, and process samples gently.

Q: RNA contamination?
A: Increase the amount of RNase A during lysis.

Q: Column clogging?
A: Reduce sample volume or improve homogenization to prevent viscous lysate.

Q: Colored eluate?
A: Use less starting material and increase the number of wash steps to remove plant pigments or phenolic compounds.

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