Human TNFa(Tumor Necrosis Factor Alpha) ELISA Kit

ELK 1190

Product name: Human TNFa(Tumor Necrosis Factor Alpha) ELISA Kit

Reactivity:Human

Alternative Names:TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

Assay Type:Sandwich

Sensitivity:6.4 pg/mL

Standard:1000 pg/mL

Detection Range:15.63-1000 pg/mL

Sample Type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length:3.5h

Research Area:Cytokine;Tumor immunity;Infection immunity;

Uniprot ID:P01375

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided 

in this kit has been pre-coated with an antibody specific to Human TNFa. Standards or samples are added to the 

appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFa. Next, Avidin 

conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate 

solution is added, only those wells that contain Human TNFa, biotin-conjugated antibody and enzyme-conjugated 

Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid 

solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration 

of Human TNFa in the samples is then determined by comparing the OD of the samples to the standard curve.