p-mHMG1tag plasmid (20 µg) [HM-301]

Plasmid p-HMG1tag is derived from plasmid p-mHMG1, and expresses mouse HMGB1 containing the HA epitope, 

for detection with anti-HA monoclonal antibody (Falciola et al. High mobility group 1 protein is not stably associated 

with the chromosomes of somatic cells. J Cell Biol 1997, 137: 19-26).

Mouse HMGB1 contains only 2 conservative substitutions with respect to human HMGB1.

When transfected in mammalian cells, this plasmid increases transiently the HMGB1 content 4- to 7-fold.

Pubblications:

The Regulatory Role of Nuclear Respiratory Factor 1 in Bladder Cancer Cells

CUL4A-mediated ZEB1/microRNA-340-5p/HMGB1 axis promotes the development of osteoporosis

Leukoreduced PRP enhanced proliferation and ECM production yet inhibited senescence, inflammation, and

 multi-differentiation potential of AFSCs by downregulating HMGB1

HSF1 enhances the attenuation of exosomes from mesenchymal stem cells to hemorrhagic shock induced 

lung injury by altering the protein profile of exosomes

The Role of Box A of HMGB1 in Enhancing Stem Cell Properties of Human Mesenchymal Cells: A Novel 

Approach for the Pursuit of Anti-aging Therapy

Determination of the Impact of High-Intensity Pulsed Electromagnetic Fields on the Release of Damage-

Associated Molecular Pattern Molecules

Afzelin induces immunogenic cell death against lung cancer by targeting NQO2

Evidence for SIRT1 Mediated HMGB1 Release From Kidney Cells in the Early Stages of Hemorrhagic Shock

The Anti-inflammatory Effects of HMGB1 Blockades in a Mouse Model of Cutaneous Vasculitis

Nonoxid-HMGB1 Attenuates Cognitive Impairment After Traumatic Brain Injury in Rats

Calogero et al. The lack of chromosomal protein HMG1 does not disrupt cell growth, but causes lethal 

hypoglycaemia in newborn mice. Nature Genet 1999, 22: 276-80.

Aidinis et al. The RAG1 homeodomain recruits HMG1 and HMG2 to facilitate Recombination Signal Sequence 

binding and to enhance the intrinsic DNA-bending activity of RAG1-RAG2. Mol Cell Biol 1999, 19: 6532-42.