MB-Si Kit II - 96 reactions - DNA/RNA isolation with magnetic beads for King Fischer

The "MB-Si Kit2" reagent kit is for using at automatic stations for the isolation of NA KingFisher Flex, Thermo 

Fisher Scientific; systems for the automatic isolation of nucleic acids from human biological samples like Auto-

Pure 96 from Hangzhou Allsheng Instruments Co., Ltd or similar.

Reagents and several 96-plates are sufficient for 96 reactions.

 The principle of the "MB-Si Kit2" reagent kit is based on the reversible binding of nucleic acid molecules on 

the surface of magnetic sorbent particles. The sample is treated with a lysis solution in the presence of magnetic 

sorbent particles. As a result, cell membranes, viral membranes and other biopolymer complexes are destroyed 

and nucleic acids are released. Dissolved NA links with the sorbent particles, while the other components of the 

lysed biological material remain in solution and are removed during magnetic precipitation of sorbent on a magnetic 

stand followed by washing of sorbent. When an elution buffer is added to a magnetic sorbent, NA is eluted from the 

surface of the sorbent into solution, which is then separated from the sorbent particles by magnetic force. As a result

of this procedure, a highly purified NA sample is obtained, free from amplification reaction inhibitors, which ensures 

high analytical sensitivity of the RT-PCR study.