GMA-001

Murine Anti- Factor IX

Clone GMA-001

Background

Factor IX (FIX) is a vitamin K-dependent zymogen that plays an essential role in the coagulation cascade leading to 

thrombus formation. In the presence of calcium, activated Factor IX (FIXa) complexes with Factor VIIIa on phospholipid 

surfaces to create the tenase complex, which converts Factor X to its activated form. Absent or defective FIX is the 

cause of the X-linked recessive bleeding disorder hemophilia B. GMA-001 (BC2) binds to the N-terminal Gla domain 

of FIX and the light chain of FIXa, as detected by Western blot and ELISA. In addition, GMA-001 prolongs clotting time 

in APTT-based plasma clotting assays and prevents arterial thrombosis in vivo in a rat model1.

Description

Species Reactivity:Human, Rat

Source:Murine

Specificity:Gla domain (residues 1-40) of FIX/FIXa

Immunogen:Human Factor IX

Isotype:IgG2a

Application

1.ELISA:Binds immobilized Human and Rat FIX, Human FIXa only in the presence of calcium.

2.Immunoblotting:Western blot detects Human FIX and light chain of Human FIXa under reduced conditions in presence of calcium.

3.Inhibition:Prolongs plasma clot time in APTT clotting assay.

Antibody References:

G.Z. Feuerstein et al. (1999). Thromb. Haemost. 82(5):1443-5.

G.Z. Feuerstein et al. (1999). Arterioscler Thromb Vasc Biol. 19(10):2554-62.

J. Tie et al. (2011). Blood. 117(10):2967-2974.

J. A. Haque et al. (2014). Blood. 123(4):582-589.

M. A. Chiasson et al. eLife. (2020) 9:1-25.