dNTP Mix - 10 mM Solution

Equimolar Mix of 10 mM dATP, dCTP, dGTP and dTTP

2'-Deoxyadenosine-5'-triphosphate, Sodium salt; 2'-Deoxycytidine-5'-triphosphate, Sodium salt; 2'-

Deoxyguanosine-5'-triphosphate, Sodium salt; 2'-Deoxythymidine-5'-triphosphate, Sodium salt

Catalog # :NU-1006S

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Molecular Formula:

dATP: C10H16N5O12P3 (free acid)

dCTP: C9H16N3O13P3 (free acid)

dGTP: C10H16N5O13P3 (free acid)

dTTP: C10H17N2O14P3 (free acid)

Molecular Weight:

dATP: 491.18 g/mol (free acid)

dCTP: 467.15 g/mol (free acid)

dGTP: 507.18 g/mol (free acid)

dTTP: 482.17 g/mol (free acid)

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution

pH: 8.5 ±0.2 (22 °C)

Applications:

For standard PCR applications a final concentration of 200 μM each dNTP is recommended.

Description:

dNTP Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP supplied as clear aqueous solution (pH 8.5).

Quality Control Specifications:

Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template or less

RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template or less

Contamination with bacterial or human DNA: not detectable

DNases, RNases, Nicking Activity: not detectable

Proteases: not detectable

Selected References:

Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.

Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of 

Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.

Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.