AHSG

Alpha-2-HS-Glycoprotein Human

Catalogue number

PRO-418

Synonyms

Alpha-2-HS-glycoprotein, Fetuin-A, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, 

AHSG, FETUA, AHS, A2HS, HSGA, PRO2743.

Introduction

Fetuin is a liver-produced negative acute phase protein composed of two subunits, the A and B chains.

Fetuin homologs have been identified in several species including rat, sheep, pig, rabbit, guinea pig, cattle,

mouse and human. Multiple physiological roles for these homologs have been suggested, including ability 

to bind to hydroxyapatite crystals and to specifically inhibit the tyrosine kinase (TK) activity of the insulin 

receptor (IR).

Fetuin-A (alpha2-Heremans-Schmid glycoprotein; AHSG) is an important circulating inhibitor of calcification

in vivo, and is downregulated during the acute-phase response.

Sera from patients on long-term dialysis with low AHSG concentrations showed impaired ex-vivo capacity to 

inhibit CaxPO4 precipitation.

Fetuin may influence the resolution of inflammation by modulating the phagocytosis of apoptotic cells 

by macrophages.

ASHG blocks TGF-beta-dependent signaling in osteoblastic cells, and mice lacking ASHG display growth 

plate defects, increased bone formation with age, and enhanced cytokine-dependent osteogenesis.

Description

The human Alpha-2-HS-Glycoprotein containing 349 a.a was purified from the human plasma.

The total molecular mass is 37.3kDa (calculated without glycosylation).

Source

Purified from the human plasma.

Physical Appearance

Filtered White lyophilized (freeze-dried) powder.

Formulation

Filtered (0.4µm) and lyophilized from 0.5 mg/ml in 20Mm Tris buffer and 200mM NaCl, pH 8.0.

Solubility

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml 

and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an 

appropriate sterile filter before using it in the cell culture.

Stability

Store the lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. 

Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Purity

Greater than 95% as determined by SDS-PAGE.

Safety Data Sheet

SDS

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, 

agricultural or pesticidal products, food additives or household chemicals.