AI Bio 소식

2025.12.15 23:02

ANGIO-PROTEOMIE 에이아이바이오허브 cAP-0001GFP, GFP Expressing Human Umbilical Vein Endothelial Cells

  • AI바이오허브 26일 전 2025.12.15 23:02 제품소개
  • 22
    0
tBKysHkNTEgZgFckhPUkWRRhOl.png
ANGIO-PROTEOMIE 전문 대리점 AI Bio Hub
ANGIO-PROTEOMIE (cAP-0001GFP) 시약을 쉽고 빠르게 구매할 수 있는 곳을 찾고 계신가요?

AI BIO HUB
견적 문의: aibiohub@aibiohub.co.kr

GFP Expressing Human Umbilical Vein Endothelial Cells

Item#:
cAP-0001GFP
OUJghfKlwnpiefcaibjneBilFc.png
ORDER INFORMATION
Name of ProductsGFP Expressing Human Umbilical Vein Endothelial Cells (GFP-HUVECs)
Catalogue NumbercAP-0001GFP
Product FormatFrozen Vial
Cell Number>5x10[5] cells/vial
GENERAL INFORMATIONHUVECs (cAP-0001) were infected with GFP- expressing Lentiviral particles @ passage 1. Puromycin resistant GFP-HUVECs (cAP-0001GFP) were selected and shipped in frozen vials (cells are provided @ passage 3). Endothelial Growth Medium (cAP-02) is recommended for cell culture and these cells have a minimum of 18 population doubling levels when cultured following the protocols described below).
CHARACTERIZATION OF THE CELLS
1.Cytoplasmic VWF/Factor VIII >95% positive by immunofluorescence
2.Cytoplasmic uptake of Di-I-Ac-LDL >95% positive by immunofluorescence
3.Cytoplasmic PECAM1 >95% positive by immunofluorescence
4.HUVECs are negative for HIV-1, HBV, HCV, and mycoplasma
PRODUCT USAGECells are offered for Research Use Only.
SHIPPINGFrozen Vials in Dry Ice package.
HANDLING OF ARRIVING CELLSWhen you receive the dry ice package with cells in frozen vials, transfer the frozen vials of cells into a -80C freezer for short period storage or a liquid nitrogen tank for long- term storage.
PROTOCOLS FOR THAWING THE CELLS AND SUBCULTURE
A)Pre-coating of T25 flasks- Add 2ml each Quick Coating Solution (cAP-01) into a T25 flask to cover the whole surface of the flask, 5 mins later, dispose the excessive coating solution by aspiration and the flask is ready to be used (although solution containing other extracellular matrix, i.e. gelatin, collagen, and fibronectin, can be used, make sure to optimize the conditions in advance).
B)Thaw the frozen cell vial in a 37C water bath first, and then transfer the cells into the pre-coated T25 flask with 10ml of cAP-02 medium, cells usually become confluent overnight and ready to be passaged.
C)To passage the cells, rinse the cells in a T25 flask with 5ml HBSS (RT) twice; then add 2ml Trypsin/EDTA (RT) (cAP-23) into one T25 flask; gently dispose the excessive Trypsin/EDTA solution within 20 seconds by aspiration.
D)Leave the T25 flask with the cells at RT or 37C for 1 min (most cells usually will detach from the surface within 1-2 mins; or monitor the cells under a microscope until most of cells become rounded up, and then gently tap the flask against the bench surface, and the cells will move on the surface of the flask when monitoring under microscope.
E)Add 5ml Trypsin Neutralization Buffer and spin down the cells with 800RPM centrifugation for 5 mins.
G)Re-suspend the cell pellet with 10 or 15ml cAP-2 medium and transfer 5 ml each into 2 or 3 pre-coated T25 flasks (for 1/2 to 1/3 subculture ratio).
H)Change medium every 2 or 3days and the cells usually become confluent within 7 days (when split at a 1/3 ratio).
I)To prepare quiescent cells, when cells are nearly confluent, replace cAP-02 with Endothelial Basal Medium (EBM, cAP-03) containing 0.5%FBS for about 8-12hrs before your experiments.
RELATED PRODUCTS
Quick Coating SolutioncAP-01
Endothelial Growth MediumcAP-02
Endothelial Basal MediumcAP-03
HBSS w/o Ca2+, Mg2+cAP-11
Cell Freezing Solution (FBS)cAP-22
Cell Freezing Solution (Non-FBS)cAP-22B
Trypsin/EDTA SolutioncAP-23
Trypsin Neutralization SolutioncAP-28
ITS (100x)cAP-26
L-Glutamine-MAXIMUM (100x)cAP-27
Human Plasma Fibronectin SolutioncAP-42
STATEMENTHandling human tissue derived products is potentially bio-hazardous, despite tested negative for HIV, HBV and HCV DNA; nonetheless, proper precautions must be taken to avoid inadvertent exposure.
For customers using our primary cell products, we strongly advise you using our own medium, although similar cell growth medium can be purchased and used for our primary cell products.
The quality of cells, in term of their growth features stated in our datasheets, will not be guaranteed if NON-ANGIO-PROTEOMIE's Cell Growth Medium is used.
Due to the sensitive nature of primary cells and cell lines, all quality related issues about the cells products must be reported back to us within ONE month period after receiving the products, no quality warranty (i.e. replacement of cells) will be provided after the ONE-Month period. Thank you for your understanding.
SPECIAL NOTES: 1. We strongly advise our customers using our own medium and related accessory products; 2. The quality of cells, in term of their growth features stated in our datasheets, will not be waranteed if NON-ANGIO-PROTEOMIE's Cell Growth Medium is used; 3.Due to the sensitive nature of primary cells and cell lines, all quality related issues about the cells products must be reported back to us within ONE month period after receiving the products, no quality warranty (i.e. replacement of cells) will be provided after the ONE-Month period.

ANGIO-PROTEOMIE (cAP-0001GFP) 제품의 신속한 견적, 빠른 주문 확인, 까다로운 수입 문제까지 바로 처리해 드립니다.
지금 바로 AI BIO HUB의 전문 서비스를 경험해 보세요!
견적 문의: aibiohub@aibiohub.co.kr

 
#CDBioparticles #Medkoo #CreativePEGWorks #BosterBio #RuixiBiologicalCompany #BCCM #Spherotech#Fivephoton #Biomat #HaWorks #Cytodiagnostics #GreenMountainAntibodies #VitasChemical #ApolloScientific#Nanografi #Accegen #ASCA #Novo #Prolabs #LDN #Angene #ChemFaces #AffinityBioscience #DimaBiotech#ELKBiotechnology #ChengduBiopurify #ProspecBio #Biochemazone #CreativeDiagnostics #GeneOn #Biopal#Liposoma #Pronetbio #Larodan #Wantai #Solvionic #Sichem #ScrippsLabs #SangonBiotech #RappPolymere#Quickzyme #ProgenBiotechnik #Pharmacosmos #PeptideInstitute #OxfordBiomedicalResearch #OEA#OD260 #NZYtech #NittoboMedical #Neochema #NanosoftPolymers #MyBiosource #Moradec #MolecularDepot#Lumiprobe #JenKem #JenaBioscience #IoLiTec #Innoprot #Imec #Iduron #HMC #HistoLine #HelloBio#Glyconz #GenTarget #FocusBiomolecules #FineScienceTools #Fapon #Surtex #FabGennix #Enamine#Elastin #eEnzyme #DHI #CymitQuimica #Cyanagen #Biomart #CPAchem #ConjuProbe #CLS #CellLineService#ClodronateLiposomes #Clearsynth #CellomicsTechnology 
#AngeneChemical #CaroteNature #BTLab #BroadPharm#BonOpusBiosciences #BOCSciences #BLDPharmatech #Biorelevant #Biomatik #BioennoLifesciences#Biobool #BioAustralis #BBISolutions #AvivaSystemsBiology #Assaypro #AssayBioTech #AngioProteomie#AngioBio #AmidBiosciences #Aktinmicals #AcceGenBiotechnology #Abmart #Abbexa #1ClickChemistry#101Bio #Cancertool #EDQM #MilanAnalytica #Analytica #Bioron
 

  • 공유링크 복사