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2025.03.05 16:58

[ELK Biotechnology 공식 대리점] Human EPO(Erythropoietin) ELISA Kit(ELK1011)

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Overview 

Overview

Product name:Human EPO(Erythropoietin) ELISA Kit
Reactivity:Human
Alternative Names:EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
Assay Type:Sandwich
Sensitivity:11.6 pg/mL
Standard:2000 pg/mL
Detection Range:31.25-2000 pg/mL
Sample Type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:3.5h
Research Area:Cytokine;Endocrinology;Hematology;Hormone metabolism;
Uniprot ID:P01588
Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EPO in the samples is then determined by comparing the OD of the samples to the standard curve.


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