3-Azido-7-hydroxycoumarin 

Catalog Number:CLK-FA047

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C,store dark

Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C9H5N3O3

Molecular Weight: 203.15 g/mol

Exact Mass: 203.03 g/mol

Purity: ≥ 90 % (HPLC)

Form: solid

Color: brown

Solubility: DMF, DMSO, MeCN, MeOH (Remark: solution in DMSO and DMF are not suitable for longterm storage)

Spectroscopic Properties: λabs 348 nm (dye before click reaction in 100 mM Tris-HCl pH 7.5), λabs 404 nm (after click reaction with L-Homopropargylglycine in 100 mM potassium phosphate buffer pH 7.0), λem 477 nm (after click reaction with L-Homopropargylglycine in 100 mM potassium phosphate buffer pH 7.0)

Selected References:

Laughlin et al. (2009) Imaging the glycome. Proc Natl Acad Sci USA. 106 (1):12.

Li et al. (2010) Fluorogenic click reaction for labeling and detection of DNA in proliferating cells. Biotechniques. 49 (1):525.

Sivakumar et al. (2004) A fluorogenic 1,3-dipolar cycloaddition reaction of 3-azidocoumarins and acetylenes. Org Lett. 6 (24):4603.