L-Homopropargylglycine (L-HPG) 

(S)-2-Aminohex-5-ynoic acid, HCl salt

Catalog Number:CLK-1067

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C;store dry

Shelf Life: 12 months after date of delivery

Molecular Formula: C6H9NO2

Molecular Weight: 127.14 g/mol (free acid)

Exact Mass: 127.06 g/mol (free acid)

CAS#: 942518-19-6

Purity: mass identification conforms (ESI-MS)

Form: solid

Color: off-white to grey

Applications:Proteins synthesis monitoring[1,2,3]

Description:

L-Homopropargylglycine (L-HPG) provides a non-radioactive alternative to analyze the global protein synthesis in cell culture. It is cell-permeable and randomly incorporated instead of methionine during translation[1,2,3]. The resulting alkyne-labeled full-length proteins can subsequently be detected via Cu(I)-catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.

Presolski et al.[4] and Hong et al.[5] provide a general protocol for Cu(I)-catalyzed click chemistry reactions that may be used as a starting point for the set up and optimization of individual assays.