0.05-2 kbp DNA Marker 

Catalogue No: abx461023

0.05-2 kbp DNA Marker contains 15 DNA fragments ranging from 50 to 2000 base pairs. The 300, 1000 bp and 2000 bp bands have the highest intensity for easy identification. Ready-to-use, the blue dye in the loading buffer runs at approximately 4 bp on a 1% gel. Can optionally be used for mass determination. 1 test is equivalent to 1 lane.

Target:0.05-2 kbp DNA Marker

Tested Applications:SDS-PAGE

Form:Liquid

Storage:Store all components at -20°C. Avoid repeated freeze/thaw cycles.

Shelf Life:Up to 12 months.

Buffer:Contains a restriction digest plus one or more PCR products.

Availability:Shipped within 10-20 working days.

Note:THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

Directions for use:Thaw, mix, and briefly centrifuge before use. Vortex the tube, then add 5 µl of ladder per lane. There is no need to add loading buffer to the ladder. The provided 5X Sample Loading Buffer can be used for sample loading; it should not be used for diluting the ladder.

Recommended dilutions

Recommended Agarose % (w/v) Effective Separation Range of Linear DNA (bp)

   0.3          5000 - 60,000

0.5             1000 - 20,000

0.7              800 - 12,000

1.0              500 - 10,000

1.2              400 - 7000

1.5              200 - 3000

2.0              100 - 2000

2.5               50 - 100

3.0               10 - 75

Note: Circularized plasmids can be cut and linearized by an appropriate restriction enzyme to measure their molecular weight.