Endonuclease ELISA 

Catalog Number: PRNUC

Key Features

ELISA for quantitative in vitro measurement of Serratia marcescens endonucleases

Test may also be used to monitor the removal of endonucleases such as DENARASE® or Benzonase® Nuclease from process related samples for process development and quality control purposes

PRODUCT DESCRIPTION:

Quantity:96 tests

Application:ELISA

Reactivity:Serratia marcescens endonuclease

Storage:2-8°C

Intended use:Research use only

Application:ELISA

Form supplied:Reagent kit, 12 x 8-well strips

BACKGROUND

This ELISA kit is intended for the quantitative in vitro measurement of Serratia marcescens endonuclease. This test may be used to monitor the removal of endonucleases such as DENARASE® or Benzonase® Nuclease from process related samples for process development and quality control purposes. 

The ELISA principle: This Endonuclease ELISA kit is a sandwich ELISA to be performed in a microtiter plate format. A specific monoclonal capture antibody is coated onto wells of a microtiter plate and is used to capture Serratia marcescens endonuclease from samples. Captured endonucleases are detected in two steps: 

1. A biotin-conjugated monoclonal detector antibody is added. 

2. A streptavidin peroxidase conjugate reacts with the biotin molecules. 

Adding substrate solution results in a colour reaction, which is proportional to the analyse concentration present in the wells. The absorbance is measured photometrically at 450 nm (optional: reference wavelength between 620 – 690 nm). The endonuclease concentration in unknown samples can be calculated based on the corresponding DENARASE® standard curve.