Aminoallyl-dUTP-XX-ATTO-643 

5-(3-Aminoallyl)-2'-deoxyuridine-5'-triphosphate, labeled with ATTO 643, Triethylammonium salt

Catalog#:NU-803-XX-643

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C

Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C24H41N5O16P3 - ATTO 643 (free acid)

Molecular Weight: 1567.90 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in 10 mM Tris-HCl

Color: blue

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λexc 643 nm, λem 665 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5)

Applications:

Incorporation into DNA/cDNA by

- PCR with Taq polymerase in-house data

- Nick Translation with DNAse I/ DNA Polymerase I in-house data

Description:

Aminoallyl-dUTP-XX-643 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. ATTO 643 is a hydrophilic dye with excellent photostability compared to fluorescein.

Recommended Aminoallyl-dUTP-XX-643/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-XX-643/ 80-70% dTTP

Please note: Protect the Dye-labeled dUTP from exposure to light and carry out experimental procedures in low light conditions. The optimal final concentration of the Dye-labeled dUTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Dye-labeled-dUTP/dTTP ratio is recommended.

Bioz AI Summary: In the study, Aminoallyl dUTP XX ATTO 643 from Jena Bioscience played a crucial role by allowing the incorporation of fluorescent labels into RNA transcripts. This enabled the visualization and tracking of RNA within the synthetic nuclear architectures, which was essential for understanding the co-transcriptional condensation and the formation of complex condensate patterns. The ability to precisely modulate and observe these interactions significantly contributed to the study's findings on the control and dynamics of RNA condensation in synthetic nuclear environments.