Alpha-Synuclein (SNCA) Rapid ELISA Kit (96T) 

Model:hSNCA

Methods Overview:

The included ELISA micro-strip plate is provided pre-coated with an a monoclonal capture antibody selective to the antigen.  

Samples are pre-mixed with a biotin-linked detection antibody and streptavidin-HRP, which are then added to the plate.  

The plate is washed after a 60 min incubation period.  Color reagents are added, resulting in a reaction with HRP that yields

 a blue colored solution.  A stop solution is applied to terminate the reaction, and the solution is measured at 450nm. 

Introduction to Alpha-Synuclein:   Alpha-synuclein (SNCA gene) is a 140 amino acid  protein expressed in brain that is 

associated with fibrillar structures in Lewy Bodies.  Although primary located in the cytosol, alpha-synuclein binds to 

phospholipid and may assemble into pore-like structures in cellular membranes.  Generation of aggregates in Lewy 

Bodies and/or association of mutant or wild-type alpha-synuclein with membraneous structures have been attributed 

to the etiology of both early-onset congenital and age-related Parkinson’s Disease. 

Parkinson's disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular 

rigidity and postural instability.  Additional features of the disease include the characteristic postural abnormalities, 

dysautonomia, dystonic cramps, and dementia. The pathology of Parkinson disease involves the loss of dopaminergic 

neurons in the substantia nigra and the presence of Lewy bodies in brain and spinal cord. 

Gerneral References on Alpha-Synuclein

Adamczyk, A. et al. (2007) Alpha-synuclein decreases arachidonic acid incorporation into rat striatal synaptoneurosomes. 

Folia Neuropathol 45 (4), 230-235

Kim, H.J. et al. (2007) Seed-dependent accelerated fibrillation of alpha-synuclein induced by periodic ultrasonication treatment. 

J Microbiol Biotechnol 17 (12), 2027-2032

Wakabayashi, K. et al. (2007) The Lewy body in Parkinson's disease: molecules implicated in the formation and degradation of 

alpha-synuclein aggregates. Neuropathology 27 (5), 494-506

Yoshida, M. (2007) Multiple system atrophy: alpha-synuclein and neuronal degeneration. Neuropathology 27 (5), 484-493

Kloepper, K.D. et al. (2007) Solid-state NMR spectroscopy reveals that water is nonessential to the core structure of alpha-

synuclein fibrils. J Phys Chem B 111 (47), 13353-13356

Buchman, V.L. and Ninkina, N. (2008) Modulation of alpha-synuclein expression in transgenic animals for modelling 

synucleinopathies--is the juice worth the squeeze? Neurotox Res 14 (4), 329-341

Fuchs, J. et al. (2008) Genetic variability in the SNCA gene influences alpha-synuclein levels in the blood and brain. 

Faseb J 22 (5), 1327-1334

Parihar, M.S. et al. (2009) Alpha-synuclein overexpression and aggregation exacerbates impairment of mitochondrial 

functions by augmenting oxidative stress in human neuroblastoma cells. Int J Biochem Cell Biol 41 (10), 2015-2024

Gibrat, C. et al. (2009) Differences between subacute and chronic MPTP mice models: investigation of dopaminergic 

neuronal degeneration and alpha-synuclein inclusions. J Neurochem 109 (5), 1469-1482