dATP - Solution 

Catalog #:NU-1001

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C

Shelf Life: 12 months

Molecular Formula: C10H16N5O12P3 (free acid)

Molecular Weight: 491.18 g/mol (free acid)

CAS#: 1927-31-7

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution

Concentration: 100 mM - 110 mM/pH: 8.5 ±0.2 (22 °C)

Spectroscopic Properties: λmax 259 nm, ε 15.1 L mmol-1 cm-1 (Tris-HCl pH 7.0)

Description:dATP, PCR-grade is supplied as ultrapure aqueous solution (pH 8.5) and suitable for all molecular biology applications including PCR/qPCR, reverse transcription, DNA labeling and DNA sequencing.

Selected References:

Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.

Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.

Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.