AF 343 (Coumarin)-X-azide 

AF 343 (Coumarin) is a blue emitting fluorophore with an emission maximum of around 480 nm. This dye forms a FRET pair with fluorescein and can harvest blue light energy for the subsequent transfer to other fluorophores.

The azide derivative can be conjugated with alkynes in copper-catalyzed and copper-free сlick reactions. The molecule contains a long aminohexanoyl linker that provides separation between the dye and the azide function.

General properties

Appearance:yellow solid

Mass spec M+ increment:480.3

Molecular weight:480.56

Molecular formula:C25H32N6O4

IUPAC name:5-{[6-(3-Azidopropylamino)-6-oxohexylamino]carbonyl}-3-oxa-13-azatetracyclo[7.7.1.02,7.013,17]heptadeca-1,5,7,9(17)-tetraen-4-one

Solubility:good in DMF, DMSO

Quality control:NMR 1H, HPLC-MS (95%)

Storage conditions:Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation/absorption maximum, nm:437

ε, L⋅mol−1⋅cm−1:39000

Emission maximum, nm:477

Fluorescence quantum yield:0.63

CF260:0.29

CF280:0.24

견적 문의: aibiohub@aibiohub.co.kr

홈페이지: www.aibiohub.co.kr   

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