LB Broth (Auto Induction Medium) 

Dehydrated powder for the preparation of LB Broth w/o trace elements, supplemented with glucose and alpha lactose for the auto induction of protein expression under the control of IPTG-inducible promoters in Escherichia coli.

SKU:N/A

Category:Auto Induction Media

DESCRIPTION:

Dehydrated powder for the preparation of LB Broth w/o trace elements, supplemented with glucose and alpha lactose for the auto induction of protein expression under the control of IPTG-inducible promoters in Escherichia coli.

FEATURES:

Quantity: 500g

Appearance: Beige powder. Autoclaved medium should be amber.

Storage: 2ºC – 25ºC. When not in use, keep container closed to avoid hydration.

Preparation:

Add 34,85g of the dehydrated medium to one liter of distilled water. Mix well and dissolve by heating with regular agitation. Boil for 1 minute in order to dissolve completely. Dispense in appropriate containers and sterilize by autoclaving at 121ºC for 15 to 20 minutes. Store at 2ºC to 8ºC.

Formulation (g/L)

Tryptone: 10,00

Yeast Extract: 5,00

MgSO4: 0,15

(NH4) 2SO4: 3,30

KH2PO4: 6,80

Na2HPO4: 7,10

Glucose 0,50

Alpha Lactose 2,00

Final pH (25ºC): 7,0 ± 0,2

ORDER INFORMATION

GCM17.0500 LB Broth (AIM) 500g

견적 문의: aibiohub@aibiohub.co.kr
홈페이지: www.aibiohub.co.kr       

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