DH10B Chemically Competent E. coli Cells for Large Plasmids Cloning 

The DH10B chemically competent E. coli cells are suitable for cloning and propagation of large size plasmids (10-150 kb in size). DH10B strain is known for stable replication of high-copy number plasmids and demonstrates improved cloning efficiency of mammalian DNA containing methylated cytosine or adenine. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. The DH10B chemically competent E. coli cells are supplied as a pack of 10 convenient single use aliquots (100 µl/tube).

Features and Benefits:

Transformation efficiency: 1-5 x 10^8 cfu/µg pBR322 DNA

Improved cloning of large plasmids and BACs due to constitutive deoxyribose synthesis

Efficient transformation of both methylated and unmethylated DNA from genomic DNA, PCR, cDNA and many other sources due to the lack of methylation-dependent restriction systems

Increased yield and quality of plasmid preparations due to mutation of nonspecific endonuclease I (endA1)

Suitable for blue/white screening via lacZ-based α-complementation

Recombination-deficient (recA1)

Streptomycin resistant

K12 Strain

Economical price

Catalog # DH10B-201

Transformation efficiency: 1-5 x 10^8 cfu/µg pBR322 DNA

Antibiotic resistance: Streptomycin

Strain: K12

Genotype:F- mcrA ∆(mrr-hsdRMS-mcrBC) φ80lacZ∆M15 ∆lacX74 recA1 endA1 rpsL150(StrR) araD139 ∆ (ara, leu)7697 galU galK λ- nupG

Storage: at -80°C

References:

Durfee et al. The Complete Genome Sequence of Escherichia coli DH10B: Insights into the Biology of a Laboratory Workhorse. (2008) J. Bacteriol. 190 (7): 2597.

견적 문의: aibiohub@aibiohub.co.kr
홈페이지: www.aibiohub.co.kr   

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