GRS DNA Loading Buffer Green (6X) 

DNA loading Buffer Green (6x) is a pre-mixed Loading Buffer for Agarose and Polyacrylamide gel electrophoresis, containing Xylene Cyanol FF and Orange G as tracking dyes.

SKU:N/A

Categories:Buffers, DNA Ladders:GRS DNA Loading Buffer Green (6X) is a pre-mixed Loading Buffer for Agarose and Polyacrylamide gel electrophoresis, containing Xylene Cyanol FF and Orange G as tracking dyes. EDTA is included to chelate Magnesium (up to 10 mM), stopping enzymatic reactions.

Contents:Solution of 10mM Tris-HCl pH 7,6; 60mM EDTA; 0,03% Bromophenol Blue; 0.15% Orange G; 60% Glycerol.

Applications:Preparation of DNA samples and markers for loading onto agarose and polyacrylamide gels. This Loading Buffer is a 6X concentrate: add 1μl of GRS DNA Loading Buffer Green (6x) per 5 μl of DNA sample.

Dye Migration:Migration of the tracking dyes depend on the type of gel, concentration of the gel and the electrophoresis buffer. For example, on a 1.5% agarose gel the tracking dyes co-migrate with DNA fragments of the following sizes:

Xylene Cyanol FF

• TAE: ~ 2.8 kb
• TBE: ~ 1.8 kb
• VOLTage Fast: ~ 900bp

Orange G

• TAE: ~ 50bp
• TBE: ~ 50bp
• VOLTage Fast: ~ <40bp

Specifications:Free of DNases and RNases.

Quality Control:Absence of endodeoxyribonucleases, exodeoxyribonucleases, and ribonucleases was confirmed by appropriate assays.

Storage:Store at room temperature or +4ºC for up to at least 12 months. For longer storage, store at -20ºC.

ORDER INFORMATION

• GLB02.0001 GRS DNA Loading Buffer Green (6X) 1mL
• GLB02.0501 GRS DNA Loading Buffer Green (6X) 5 x 1mL
• GLB02.2001 GRS DNA Loading Buffer Green (6X) 20 x 1mL